Expressions of P53, topoisomerase Ⅱ and multi drug resistance associated protein in tissues of colorectal cancer of patients combined with chronic schistosomiasis

Objective:To investigate the expressions of P53, topoisomerase Ⅱ (Topo Ⅱ) and multidrug resistance associated protein (MRP) in tissues of colorectal cancer of patients combined with chronic schistosomiasis.
Method: The retrospective casecontrol study was adopted. The clinicopathological data of 338 colorectal cancer patients who were admitted to the Zhejiang Cancer Hospital between January 2008 and December 2010 were collected. Cancer tissue specimens from surgical resection were collected. Among 338 patients, 80 were combined with chronic schistosomiasis and 258 were combined with nonchronic schistosomiasis. The expressions of P53, Topo Ⅱ and MRP were dectected using immunohistochemistry (IHC). Ranked data were presented as percentage and analyzed using the nonparametric test.
Results:The negative, weak positive, positive and strong positive expressions of P53 were respectively 5.00%(4/80), 87.50%(70/80), 3.75%(3/80), 3.75% (3/80) in tissues of colorectal cancer of patients combined with chronic schistosomiasis and 28.68%(74/258), 19.38%(50/258), 16.67%(43/258), 35.27%(91/258) in tissues of colorectal cancer of patients combined with nonchronic schistosomiasis, with a statistically significant difference (Z=-2.962, P<0.05). The negative,weak positive, positive and strong positive expressions of Topo Ⅱ were respectively 8.75%(7/80), 51.25%(41/80), 22.50%(18/80), 17.50%(14/80)in tissues of colorectal cancer of patients combined with chronic schistosomiasis and 12.01%(31/258), 55.43%(143/258), 22.48%(58/258), 10.08%(26/258) in tissues of colorectal cancer of patients combined with nonchronic schistosomiasis, with no statistically significant difference (Z=-1.551, P>0.05). The negative, weak positive, positive and strong positive expressions of MRP were respectively 7.50%(6/80), 40.00%(32/80), 28.75%(23/80), 23.75%(19/80)in issues of colorectal cancer of patients combined with chronic schistosomiasis and 24.42%(63/258), 38.37%(99/258), 24.03%(62/258), 13.18%(34/258) in tissues of colorectal cancer of patients combined with nonchronic schistosomiasis, with a statistically significant difference (Z=-3.408, P<0.05).
Conclusion:There are abnormal expressions of P53 and MRP in tissues of colorectal cancer of patients combined with chronic schistosomiasis, which may be involved in the hypothetical mechanism of chronic schistosomiasis inducing carcinogenesis of colorectal cancer.