Modified orthotopic segmental small bowel transplantation in rats

Objective　To establish a simple and stable orthotopic segmental small bowel transplantation model in rats. Methods　Eighty male SD rats were divided into donors and recipients. Orthotopic segmental small bowel transplantation was performed by endtoside anastomosis between donor　abdominal aorta tundish shape patch with arteria mesenterica superior pedicle　 and abdominal aorta of the recipients; endtoend anastomosis between the portal vein of the donors and the left renal vein of the recipients was done using the cuff technique; the large part of the small bowel of the recipients was excised, and it was replaced by the segmental intestine of the donors. Results　The operation time of the donors and recipients were (40±5)minutes and (50±8)minutes, respectively. The warm ischemia time and cold ischemia time were (5±2)minutes and (15±5)minutes, respectively. The anastomosis time of arteries and veins were　(5±2)minutes and (4±2)minutes, respectively. The survival time of 90.0%(36/40) of rats was more than 10 days. Conclusion　The modified rat model of orthotopic segmental small bowel transplantation is easy to manipulate, and has the advantages of short operation time, high survival rate and stability.