Anti tumor efficiency of cytotoxic T lymphocyte induced by activated B lymphocyte after hepatocellular carcinoma alpha fetoprotein mRNA transfection

Objective To investigate the antitumor efficiency of cytotoxic Tlymphocyte (CTL) induced by activated B lymphocyte after hepatocellular carcinoma (HCC) alpha fetoprotein (AFP) mRNA transfection.
Methods B lymphocytes were fractionated, purified and activated by recombinant human soluble sCD40L. PGEM4Z/AFP/A64EGFP plasmid was established 〖WTBX〗in vitro,〖WTBZ〗 mixed with polymerase T7RNA, and then transcribed into AFP mRNA with Poly(A) sequence. B lymphocytes electrotransfected with AFP mRNA were in the experimental group, B lymphocytes electrotransfected with GAPDH mRNA were in the negative control group, and untreated B lymphocytes were in the blank control group. The expressions of antigenpresenting cell (APC) markers (CD19, CD20, CD21, CD40, CD80, CD83) and major histocompatibility complex (MHC) of the 3 groups were detected. B lymphocytes of the 3 groups were cultured with T lymphocytes at ratios of 1∶ ±4% of the blank control group (〖WTBX〗t=9.141, 13.272, 11.901; 14.372, 12.835, 9.507, P〖WTBZ〗<0.01).
Conclusion Activated B lymphocytes after HCC AFP mRNA transfection may effectively induce CTL to kill HCC cells.