Abstract: The development of laparoscopic techniques has made surgical treatment enter into the era of minimally invasive surgery, and the application of threedimensional (3D) highdefinition laparoscopic system has brought the important innovation for laparoscopic techniques, meanwhile, it also provides stereoscopic visions and more accurate spatial orientation for surgeons. During the process of laparoscopic radical resection of lower rectal cancer, 3D vision can help to identify blood vessels, nerve and tissues around the rectum, ensure accurate lymph node dissection, reduce difficulty of operation, and the learning curve of laparoscopic radical resection of lower rectal cancer for inferior surgeons might be shorten by using 3D imaging system. With the development of technology, the value on clinical application of 3D highdefinition laparoscope will become more evident.