P53与Ⅱ型拓扑异构酶及多重耐药相关蛋白在合并慢性血吸虫病结直肠癌组织中的表达
Expressions of P53, topoisomerase Ⅱ and multi drug resistance associated protein in tissues of colorectal cancer of patients combined with chronic schistosomiasis
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摘要:
目的:探讨P53蛋白、Ⅱ型拓扑异构酶(Topo Ⅱ)及多重耐药相关蛋白(MRP)在合并慢性血吸虫病与非血吸虫病结直肠癌患者中的表达。
方法:采用回顾性病例对照研究方法。收集2008年1月至2010年12月浙江省肿瘤医院收治的338例结直肠癌患者的临床病理资料。收集患者手术切除的结直肠癌组织。338例患者中，80例合并慢性血吸虫病，258例不合并慢性血吸虫病。采用免疫组织化学染色检测结直肠癌组织中P53、Topo Ⅱ、MRP蛋白表达。等级资料以百分比表示，采用非参数检验。
结果:P53蛋白阴性、弱阳性、阳性、强阳性表达率在合并慢性血吸虫病的结直肠癌组织中分别为5.00%(4/80)、87.50%(70/80)、3.75%(3/80)、3.75%(3/80)，在不合并慢性血吸虫病的结直肠癌组织中分别为28.68% (74/258)、19.38%(50/258)、16.67%(43/258)、35.27%(91/258)，两者比较，差异有统计学意义(Z= -2.962，P<0.05)。Topo Ⅱ蛋白阴性、弱阳性、阳性、强阳性表达率在合并慢性血吸虫病的结直肠癌组织中分别为8.75%(7/80)、51.25%(41/80)、22.50%(18/80)、17.50%(14/80)，在不合并慢性血吸虫病的结直肠癌组织中分别为12.01%(31/258)、55.43%(143/258)、22.48%(58/258)、10.08%(26/258)，两者比较，差异无统计学意义(Z=-1.551，P>0.05)。MRP蛋白阴性、弱阳性、阳性、强阳性表达率在合并慢性血吸虫病的结直肠癌组织中分别为7.50%(6/80)、40.00%(32/80)、28.75%(23/80)、23.75%(19/80)，在不合并慢性血吸虫病的结直肠癌组织中分别为24.42%(63/258)、38.37%(99/258)、24.03%(62/258)、13.18%(34/258)，两者比较，差异有统计学意义(Z=-3.408，P<0.05)。
结论:P53蛋白和MRP蛋白在合并慢性血吸虫病的结直肠癌组织中均呈异常表达，提示其可能参与慢性血吸虫病诱导结直肠癌的分子机制。Abstract:Objective:To investigate the expressions of P53, topoisomerase Ⅱ (Topo Ⅱ) and multidrug resistance associated protein (MRP) in tissues of colorectal cancer of patients combined with chronic schistosomiasis.
Method: The retrospective casecontrol study was adopted. The clinicopathological data of 338 colorectal cancer patients who were admitted to the Zhejiang Cancer Hospital between January 2008 and December 2010 were collected. Cancer tissue specimens from surgical resection were collected. Among 338 patients, 80 were combined with chronic schistosomiasis and 258 were combined with nonchronic schistosomiasis. The expressions of P53, Topo Ⅱ and MRP were dectected using immunohistochemistry (IHC). Ranked data were presented as percentage and analyzed using the nonparametric test.
Results:The negative, weak positive, positive and strong positive expressions of P53 were respectively 5.00%(4/80), 87.50%(70/80), 3.75%(3/80), 3.75% (3/80) in tissues of colorectal cancer of patients combined with chronic schistosomiasis and 28.68%(74/258), 19.38%(50/258), 16.67%(43/258), 35.27%(91/258) in tissues of colorectal cancer of patients combined with nonchronic schistosomiasis, with a statistically significant difference (Z=-2.962, P<0.05). The negative,weak positive, positive and strong positive expressions of Topo Ⅱ were respectively 8.75%(7/80), 51.25%(41/80), 22.50%(18/80), 17.50%(14/80)in tissues of colorectal cancer of patients combined with chronic schistosomiasis and 12.01%(31/258), 55.43%(143/258), 22.48%(58/258), 10.08%(26/258) in tissues of colorectal cancer of patients combined with nonchronic schistosomiasis, with no statistically significant difference (Z=-1.551, P>0.05). The negative, weak positive, positive and strong positive expressions of MRP were respectively 7.50%(6/80), 40.00%(32/80), 28.75%(23/80), 23.75%(19/80)in issues of colorectal cancer of patients combined with chronic schistosomiasis and 24.42%(63/258), 38.37%(99/258), 24.03%(62/258), 13.18%(34/258) in tissues of colorectal cancer of patients combined with nonchronic schistosomiasis, with a statistically significant difference (Z=-3.408, P<0.05).
Conclusion:There are abnormal expressions of P53 and MRP in tissues of colorectal cancer of patients combined with chronic schistosomiasis, which may be involved in the hypothetical mechanism of chronic schistosomiasis inducing carcinogenesis of colorectal cancer.-
Keywords:
- Colorectal neoplasms /
- Schistosomiasis /
- chronic /
- P53 /
- Topoisomerase Ⅱ /
- Multi drug resistance associated protein
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