胆囊癌组织中Krüppel样因子8蛋白的表达及意义

Expression and significance of Krüppel like factor 8 protein in the gallbladder cancer tissues

    摘要
  • 摘要: 目的:探讨胆囊癌组织中Krüppel样因子8(KLF8)蛋白的表达及其与胆囊癌患者临床病理特征和预后的关系。
    方法:回顾性分析2008年1月至2012年12月上海交通大学医学院附属新华医院收治的40例胆囊癌患者的临床资料，采用免疫组织化学染色和Western blot法检测40例胆囊癌患者癌组织及相应癌旁组织中KLF8蛋白的表达情况，分析其与胆囊癌患者临床病理因素及预后之间的关系。采用门诊及电话方式进行随访，随访时间截至2013年11月。计数资料比较采用Pearson χ2检验、矫正χ2检验和Fisher确切概率法，计量资料比较采用t检验。采用Kaplan Meier法绘制生存曲线，Log rank法检验总体生存率的差异。
    结果:KLF8蛋白的表达主要定位于细胞核。KLF8蛋白在胆囊癌患者癌组织及癌旁组织中的阳性表达率分别为77.5%(31/40)和27.5%(11/40)，两者比较，差异有统计学意义(χ2=6.580，P<0.05)。Western blot分析结果显示：KLF8蛋白在胆囊癌患者癌组织及癌旁组织中的相对表达量分别为0.67±0.03和0.22±0.12，两者比较，差异有统计学意义(t=8.660，P<0.05)。胆囊癌组织中KLF8蛋白的阳性表达率在胆囊癌患者的性别、年龄、淋巴结转移和胆囊结石方面比较，差异无统计学意义(χ2=0.755，0.227，0.029，0.062，P>0.05)；而在肿瘤的TNM分期和分化程度方面比较，差异有统计学意义(χ2=8.027，P<0.05)。40例获得随访患者中，KLF8蛋白表达阴性的胆囊癌患者中位生存时间为19个月，3年累积生存率为44.4%；KLF8蛋白表达阳性的胆囊癌患者中位生存时间为6个月，3年累积生存率为22.6%，两者比较，差异有统计学意义(χ2=11.837，P<0.05)。
    结论:KLF8在胆囊癌组织中表达升高，这与胆囊癌的发生、发展密切相关，可作为判断患者预后的指标。

     

    Abstract: Objective:To investigate the expression of Krüppel like factor 8( KLF8 ) protein in the gallbladder cancer tissues and its relationship with the clinical pathological features and prognosis of patients.
    Methods:The clinical data of 40 patients with gallbladder cancer were analyzed retrospectively. All the patients were admitted at the Xinhua Hospital of the Shanghai Jiaotong University from January 2008 to December 2012. The expressions of KLF8 protein in the gallbladder and paracarcinoma tissues were examined by the immunohistochemistry and the Western blot. Patients were followed up through outpatient examination and telephone interview until November 2013. Count data were analyzed using the Pearson χ2 test and Fisher′s exact probability. Quantitative data were analyzed using the t test. Survival curve was generated using the Kaplan Meier method, and the survival analysis was conducted using the Log rank test.
    Results:The expression of the KLF8 protein was predominantly detected in the nuclei of the tumor cells. The positive rate of the KLF8 protein expression in gallbladder cancer and paracarcinoma tissues were 77.5%(31/40) and 27.5%(11/40), respectively, which showing a significant difference (χ2=6.580, P<0.05). The expression of KLF8 protein by Western blot method in gallbladder cancer tissues was 0.67±0.03, which were compared with 0.22±0.12 in paracarcinoma tissue, showing a significant difference (t=8.660, P<0.05). The expression level of the KLF8 protein in patients with gallbladder cancer was however not associated with gender, age, lymph node metastasis and gallstone (χ2=0.755, 0.227, 0.029, 0.062, P>0.05), however was positively correlated to TNM stage and tumor differentiation (χ2=8.027, P<0.05). The median survival time and 3 year cumulative survival rate was 19 months and 44.4% for patients with negative expression of KLF8 protein，compared with 6 months and 22.6% for those with positive expression of KLF8 protein ,with statically significance for two proteins (χ2=11.837, P<0.05).
    Conclusion:The overexpression of the KLF8 protein in the gallbladder cancer is positively correlated with the occurrence and development of gallbladder cancer, and it may be an prognostic indicator for patients.

     

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