载脂蛋白B基因XbaⅠ和EcoRⅠ位点稀有等位基因与胆石症的关系

Relationship between apolipoprotein B gene Xba Ⅰ and EcoRⅠ polymorphisms and cholelithiasis

    摘要
  • 摘要: 目的：探讨载脂蛋白B基因XbaⅠ、EcoRⅠ位点稀有等位基因与内蒙古中西部地区汉族和蒙古族胆石症人群的相关性。
    方法：收集2010年4～10月包头医学院第一附属医院100例胆石症患者(结石组)及同期行健康体检的115例正常人(对照组)的临床资料，采用病例对照研究方法和聚合酶链反应-限制性片断长度多态性(PCR-RFLP)技术，检测和分析内蒙古中西部地区的汉族和蒙古族结石组和对照组血样的载脂蛋白B基因XbaⅠ、EcoRⅠ多态性，包括XbaⅠ位点的X+X+、X+X-、X-X-基因型及等位基因X-、X+(稀有等位基因)；EcoRⅠ位点的E+E+、E+E-、E-E-基因型及等位基因E+、E-(稀有等位基因)。检测各组的血脂水平，包括TG、TC、HDL和LDL。计数资料行χ2检验，计量资料行t检验。
    结果: 汉族人群与蒙古族人群均无X+X+基因型，且蒙古族人群中亦未发现稀有等位基因X+和E-的存在。在汉族人群中，结石组LDL为(2.8±0.9)mmol/L，显著高于对照组的(1.9±0.8)mmol/L(t=2.800，P<0.05)；蒙古族人群中，结石组HDL、LDL分别为(1.7±0.3)mmol/L、(3.5±0.8)mmol/L，显著高于对照组的(1.2±0.3)mmol/L、(2.8±0.9)mmol/L(t=7.596，2.549，P<0.05)。蒙古族人群中，结石组TG、TC、HDL、LDL分别为(3.1±1.6)mmol/L、(5.6±1.0)mmol/L、(1.7±0.3)mmol/L、(3.5±0.8)mmol/L，均显著高于汉族结石组的(1.2±0.6)mmol/L、(4.4±1.2)mmol/L、(1.3±0.3)mmol/L、(2.8±0.9)mmol/L(t=5.501，3.667，4.448，3.430，P<0.05)。蒙古族对照组TG、TC、LDL分别为(2.6±1.7)mmol/L、(5.1±1.1)mmol/L、(2.8±0.9)mmol/L，均显著高于汉族对照组的(1.3±0.7)mmol/L、(3.9±0.9)mmol/L、(1.9±0.8)mmol/L(t=4.298，4.772，3.888，P<0.05)，而HDL则在汉族对照组中较高(t=1.997，P<0.05)。汉族结石组X+X-、X-X-基因型LDL水平分别为(2.7±0.1)mmol/L和(2.6±1.0)mmol/L，E+E+、E+E-/E-E-分别为(2.6±1.0)mmol/L和(2.5±0.4)mmol/L，组内比较，差异无统计学意义(t=0.225，0.124，P>0.05)。
    结论：在内蒙古中西部地区，蒙古族人群可能比汉族人群容易患胆石症，但载脂蛋白B基因XbaⅠ和EcoRⅠ位点稀有等位基因X+和E-与血脂水平增高未见关联，可能与胆石症发生无关。

     

    Abstract: Objective:To investigate the relationship between the apolipoprotein B (ApoB) gene XbaⅠ and EcoRⅠ polymorphisms and cholelithiasis in Han and Mongolian population in the Midwest Area of Inner Mongolia.
    Methods:The clinical data of 100 patients with cholelithiasis and 115 healthy individuals at the First Affiliated Hospital of Medical College of Baotou from April to October in 2010 were collected. A case-control study which detected ApoB alleles of patients with cholelithiasis (cholelithiasis group) and healthy individuals (control group) in Han nationality and Mongolian nationality in the Midwest Area of Inner Mongolia was conducted by polymerase chain reaction-restriction fragment length polymorphism, which included XbaⅠ (X+X+, X+X-, X-X-, X+, X-) and EcoRⅠ (E+E+, E-E-, E+E-, E+, E-). The serum lipid (including triglyceride, total cholesterol, high density lipoprotein and low density lipoprotein) levels in different groups were detected. The count data and the measurement data were analyzed using the chi-square test and t test, respectively.
    Results: Genotype X+X+ was not found in the Han and Mongolian population, and XbaⅠ (X+) or EcoRⅠ (E-) alleles was not found in the Mongolian population. The levels of low density lipoprotein were 〖HJ*4〗(2.8±0.9)mmol/L in the cholelithiasis group, which was significantly higher than (1.9±0.8)mmol/L of the control group in the Han population (t=2.800, P<0.05). The levels of high density lipoprotein and low density lipoprotein were (1.7±0.3)mmol/L and (3.5±0.8)mmol/L of the cholelithiasis group, which were significantly higher than (1.2±0.3)mmol/L and (2.8±0.9)mmol/L of the control group in the Mongolian population (t=7.596, 2.549, P<0.05). The levels of triglyceride, total cholesterol, high density lipoprotein and low density lipoprotein of the cholelithiasis group in the Mongolian population were (3.1±1.6)mmol/L, (5.6±1.0)mmol/L, (1.7±0.3)mmol/L and (3.5±0.8)mmol/L, which were significantly higher than (1.2±0.6)mmol/L, (4.4± 1.2)mmol/L, (1.3±0.3)mmol/L and (2.8±0.9)mmol/L of the cholelithiasis group in the Han population (t=5.501, 3.667, 4.448, 3.430, P<0.05). The levels of triglyceride, total cholesterol, low density lipoprotein were (2.6±1.7)mmol/L, (5.1±1.1)mmol/L and (2.8±0.9)mmol/L of the control group in the Mongolian population, which were significantly higher than (1.3±0.7)mmol/L, (3.9±0.9)mmol/L and (1.9±0.8)mmol/L of the control group in the Han population (t=4.298, 4.772, 3.888, P<0.05), while the level of high density lipoprotein was significantly higher of the control group in the Han nationality than the control group in the Mongolian population (t=1.997, P<0.05). The levels of low density lipoprotein in patients with genotypes X+X-, X-X- of the cholelithiasis group in the Han population were (2.7±0.1)mmol/L and (2.6±1.0)mmol/L, and the levels of low density lipoprotein in patients with genoeypes E+E+, E+E-/E-E- were (2.6±1.0)mmol/L and (2.5±0.4)mmol/L, with no significant difference (t=0.225, 0.124, P>0.05).
    Conclusion:In the Midwest area of Inner Mongolia, the Mongolian population might be more susceptible to cholelithiasis than the Han population. No relationship between the rare alleles X+, E- and the increase of blood lipids, which indicates that X+ and E- of ApoB may not be a risk factor of cholelithiasis.

     

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