miR-155在结肠癌组织中的表达及其临床意义

Expression of miR -155 and its clinical significance in tissues of colonic cancer

    摘要
  • 摘要:
    目的  研究miR-155在结肠癌组织中的表达并探讨其表达与结肠癌临床病理特征的关系。
    方法  收集2011年3月至9月襄阳市中心医院手术切除的原发性结肠癌标本57例，分别提取结肠癌和正常黏膜组织中总RNA并行逆转录反应，应用实时荧光逆转录聚合酶链反应检测肿瘤组织及正常黏膜组织中miR-155的表达水平，探讨其表达与结肠癌临床病理参数间的关系。配对标本采用非参数Wilcoxon秩和检验，两独立样本采用u检验。
    结果  miR-155在结肠癌组织中相对表达水平为0.421(0.016～1.241)，显著高于其在正常黏膜组织中表达的0.128(0.000～0.337)，两者比较，差异有统计学意义(u=3.783，P<0.05)；miR-155的表达增高与TNM分期、肿瘤浸润程度、淋巴结转移、肿瘤分化程度明显相关(u=2.364，2.152，2.338，2.214，P<0.05)。
    结论  miR-155在结肠癌组织中表达增高，miR-155可能作为原癌基因参与结肠癌的发生、发展，与结肠癌的侵袭、转移密切相关。

     

    Abstract:
    Objective  To investigate the expression of miR-155 in the tissues of colonic cancer, and the relationship between miR-155 and the clinical pathological parameters of colonic cancer.
    Methods  Fifty-seven samples of primary colonic cancer tissues and adjacent normal tissues were collected from Xiangyang Center Hospital from March to September, 2011. The total RNAs were extracted from the colonic cancer tissues and normal tissues to conduct the reverse transcription reaction. The expressions of miR-155 in the colonic cancer tissues and normal tissues were detected using real time quantitative reverse transcription-polymerase chain reaction, and the relationship between the expression of miR-155 and the clinical pathological parameters of colonic cancer was analysed. Paire samples were analyzed using non-parametric Wilcoxon test, and independent samples were analyzed using the  u test.
    Results  The relative expression of miR-155 in the colonic cancer tissues was 0.421 (range, 0.016-1.241), which was significantly higher than  0.128 (range, 0.000-0.337) in the normal tissues (u=3.783, P<0.05). The increase of miR-155 expression was correlated with TNM staging, tumor invasion, lymphatic metastasis and tumor cell differentiation (u=2.364, 2.152, 2.338, 2.214, P<0.05).
    Conclusions  The expression of miR-155 is up-regulated in colonic cancer tissues. MiR-155 may play an important role in the invasion and metastasis of human colonic cancer.

     

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