大鼠原位节段小肠移植模型的改进
Modified orthotopic segmental small bowel transplantation in rats
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摘要:
目的 建立一种简便、稳定的大鼠原位节段小肠移植模型。方法 供、受体均为雄性SD大鼠，各40只，采用供体肠系膜上动脉腹主动脉漏斗状袖片与受体肾下腹主动脉端侧吻合，供体门静脉与受体左肾静脉Cuff套管袖套吻合。切除受体大部分小肠，供体小肠近、远端分别与受体残留小肠近、远端行端端吻合。结果 供体手术时间(40±5)min，受体手术时间(50±8)min。热缺血时间(5±2)min，冷缺血时间(15±5)min。动脉吻合时间(5±2)min，静脉吻合时间(4±2)min。90.0%(36/40)的大鼠术后存活时间>10 d。结论 该模型操作简便，手术时间短，模型成活率高，稳定性好。
Abstract:Objective To establish a simple and stable orthotopic segmental small bowel transplantation model in rats. Methods Eighty male SD rats were divided into donors and recipients. Orthotopic segmental small bowel transplantation was performed by endtoside anastomosis between donor abdominal aorta tundish shape patch with arteria mesenterica superior pedicle and abdominal aorta of the recipients; endtoend anastomosis between the portal vein of the donors and the left renal vein of the recipients was done using the cuff technique; the large part of the small bowel of the recipients was excised, and it was replaced by the segmental intestine of the donors. Results The operation time of the donors and recipients were (40±5)minutes and (50±8)minutes, respectively. The warm ischemia time and cold ischemia time were (5±2)minutes and (15±5)minutes, respectively. The anastomosis time of arteries and veins were (5±2)minutes and (4±2)minutes, respectively. The survival time of 90.0%(36/40) of rats was more than 10 days. Conclusion The modified rat model of orthotopic segmental small bowel transplantation is easy to manipulate, and has the advantages of short operation time, high survival rate and stability.
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