SonicHedgehog信号通路与慢性胰腺炎的关系

Relationship between the sonic hedgehog signal pathway and chronic pancreatitis

    摘要
  • 摘要:
    目的研究SonicHedgehog(SHH)信号通路与慢性胰腺炎的关系｡方法将30只Wistar大鼠按照随机数字表法分为模型组､实验对照组和空白对照组,每组10只｡模型组大鼠的胰管内注入三硝基苯磺酸,以诱发大鼠胰腺炎｡实验对照组大鼠的胰管内注入生理盐水,空白对照组大鼠不作任何处理｡模型建立后分别于不同时间点检测3组大鼠血清中的TBil和淀粉酶水平,观察大鼠胰腺的组织学变化｡用免疫组织化学法检测patched1(PTCH1)､smoothened(SMO)和SHH的表达情况｡多组比较采用方差分析,组间比较采用Student′st检验｡结果模型组､实验对照组､空白对照组大鼠第1~35天的血清TBil水平分别为(3.17±0.21)μmol/L~(9.28±2.15)μmol/L､(3.44±0.54)μmol/L~(3.76±0.15)μmol/L､(2.45±0.32)μmol/L~(3.27±1.43)μmol/L,血清淀粉酶水平分别为(1124±143)U/L~(1522±169)U/L､(1123±104)U/L~(1007±141)U/L､(1014±115)U/L~(1013±142)U/L｡与实验对照组和空白对照组比较,模型组大鼠的血清TBil和淀粉酶水平在7~35d显著升高(F=14.81､16.27､18.13､11.49､17.91,11.54､10.24､12.64､21.11､15.38,P<0.05),胰腺组织纤维组织增生在第35天内形成;模型组大鼠的胰腺组织中PTCH1､SMO和SHH的表达显著增强｡结论 SHH信号通路在大鼠胰腺炎组织中呈活化状态,可能在胰腺炎的发生过程中发挥重要作用｡

     

    Abstract:
    Objective To investigate the relationship between sonic hedgehog (SHH) signaling pathway and chronic pancreatitis.
    Methods Thirty Wistar rats were randomly divided into the model group, experimental control group and blank control group, and there were 10 rats in each group. Trinitrobenzene sulfonic acid was  infused into the pancreatic duct of the rats to induce pancreatitis in the model group. The pancreatic duct of the rats in the experimental control group were infused with 0.9% sodium chloride solution. No treatment was  performed on rats in the blank control group. The serum levels of bilirubin and amylase of the 3 groups were determined, and the histopathological alterations were studied. Expressions of patched-1 (PTCH-1), smoothened (SMO) and SHH were detected by immunohistochemistry. All data were analyzed using the analysis of variance or Student′s  t test.
    Results The levels of total bilirubin (TBil) in the model group, experimental control group and blank control group from day 1 to day 35 were (3.17±0.21)μmol/L-(9.28±2.15)μmol/L, (3.44±0.54)μmol/L-(3.76±0.15)μmol/L, (2.45±0.32)μmol/L-(3.27±1.43)μmol/L, respectively. The levels of amylase in the model group, experimental control group and blank control group were (1124±143)U/L-(1522±169)U/L, (1123±104)U/L-(1007±141)U/L, (1014±115)U/L-(1013±142)U/L, respectively. Compared with the experimental group and the blank control group, the serum levels of TBil and amylase in the model group were  increased significantly from day 7 to day 35 F=14.81, 16.27, 18.13, 11.49, 17.91; 11.54, 10.24, 12.64, 21.11, 15.38, P <0.05), and fibrotic proliferation of pancreatic tissues were found at day 35; the expressions of PTCH-1, SMO and SHH in the pancreatic tissue was increased significantly in the model group.
    Conclusion SHH signaling pathway is activated in the tissue of pancreatitis in rats, it might play an important role in the genesis of pancreatitis.

     

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