Abstract: Gastrointestinal tumors are among the most common malignant tumors of the digestive tract in China, and their incidence is on the rise. To standardize the use of nanocarbon tracing technology in gastrointestinal tumor surgeries, the Editorial Board of the Chinese Journal of Digestive Surgery has convened a group of domestic experts in related fields. They have developed the "Expert Consensus on the Application and Operation of Nanocarbon Tracing Technology in Gastrointestinal Tumor Surgery (2025 Edition)". This consensus outlines the application scenarios and methods for utilizing nanocarbon tracing technology in the diagnosis and treatment of gastro-intestinal tumors, as well as in surgical procedures and evidence-based medicine. The primary goals are to standardize clinical application scenarios and usage methods for this technology, ultimately enhancing service for both clinicians and patients.